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  • 产品名称: Arf6 Activation Assay Kit
  • 产品货号: 82401
  • 货期: 现货
  • 价格与订购: 4800
  • 数量:
    库存: 5
  • 规格: 96T
  • 产品信息
  • 如何订购
    Product Description
     ARF6 (ADP-ribosylation factor 6) is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and functions as a regulatory molecule of phagocytosis.
        Currently there is no direct assay to measure the activation of Arf6 GTPase.
       Bioyears Biosciences Arf6 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Arf6 GTP, but not Arf6-GDP, and an Arf6 specific rabbit polyclonal antibody. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
        These anti Arf6-GTP monoclonal antibodies can also be used to monitor the activation of Arf6 in cells and in tissues by immunohistochemistry.
        Bioyears Biosciences Arf6 Activation Assay Kit provides a simple and fast method to monitor the activation of Arf6. Each kit provides sufficient quantities to perform 20 assays.
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    Assay Principle
    Bioyears Biosciences Arf6 Activation Assay Kit bases on the configuration-specific anti Arf6 GTP monoclonal antibody to measure the active Arf6 GTP levels, either from cell extracts or from in vitro GTPγS loading Arf6 activation assays. Briefly, anti active Arf6 mouse monoclonal antibody will be incubated with cell lysates containing Arf6 GTP. The bound active Arf6 will then be pulled down by protein A/G agarose. The precipitated active Arf6 will be detected by immunoblot analysis using an anti Arf6 rabbit polyclonal antibody, respectively.
    Kit Components
    1. Anti active Arf6, Mouse Monoclonal Antibody (Catalog No. 26918): One vial – 22 µL (1
        mg/ml) in PBS, pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibody
        specifically recognizes Arf6 GTP from all vertebrates.
    2. Protein A/G Agarose (Catalog No. 30301): One vial – 400 µL of 50% slurry. 
    3. 5X Assay/Lysis Buffer (Catalog No. 30302): One bottle – 30 mL of 250 mM Tris-HCl, pH 8,    750mM NaCl, 50 mM MgCl2, 5 mM EDTA, 5% Triton X-100.
    4. Anti Arf6, Rabbit Polyclonal Antibody (Catalog No. 21032): One vial – 100 µL (1 mg/ml)
        in PBS, pH 7.4, contained 50% glycerol.
    5. 100 X GTPγS (Catalog No. 30303): One vial –100 µl at 10 mM, use 5 µL of GTPγS for GTP-abeling of 0.5 mL of cell lysate.
    6. 100 X GDP (Catalog No. 30304): One vial –100 µl at 100 mM, use 5 µL of GDP for GDP-    labeling of 0.5 mL of cell lysate. 
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    Storage
    Store all kit components at 4ºC until their expiration dates. 
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    Note
    For Research Use Only.