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  • 产品名称: Anti SMAD4(D351H) Mouse Monoclonal Antibody
  • 产品货号: CST26418
  • 货期: 现货
  • 价格与订购: 4800
  • 数量:
    库存: 5
  • 规格: 100μg
  • 产品信息
  • 如何订购
    Catalog Number
    CST26418
    Gene Symbol
    SMAD4, DPC4, JIP, MADH4, MYHRS
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    Description
    Anti SMAD4(D351H) Mouse Monoclonal Antibody
    Background
    SMAD4 family member 4, also known as SMAD4, is a protein that in human is encoded by the SMAD4 gene. It is often found mutated in many cancers. It acts as a tumor suppressor that functions in the regulation of the TGF-? signal transduction pathway, which negatively regulates growth of epithelial cells and the extracellular matrix (ECM). SMAD4 alterations have been found in multiploid colorectal cancer and pancreatic carcinoma. It is found inactivated in at least 50% of pancreatic cancers. It is also found mutated in the autosomal dominantdisease juvenile polyposis syndrome (JPS).
    Immunogen
    A synthetic peptide from the internal region of SMAD4 which includes the mutation of D351H, human origin.
    Applications
    ELISA, WB, IHC
    Recommended Dilutions
    ELISA: 1:1000-1:2000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration
    1 mg/ml
    Host
    mouse
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    Format
    Liquid
    Clonality
    Monoclonal
    Purity
    Purified from ascites
    Preservative
    no
    Constituents
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity
    Recognizes D351H mutant, but not wild-type SMAD4 of vertebrates.
    Immunoprecipitation/Western Blot

    Western blot analysis of recombinant SMAD4(D351H) and wildtype proteins. Purified His-tagged SMAD4(D351H) protein (lane1) and corresponding wild-type protein (1-552aa, 65kd) (lane2) were blotted with anti SMAD4(D351H) monoclonal antibody (Cat. #26418).   
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    Immunofluorescence: 

    Immunofluorescence of cells expressing SMAD4 proteins with anti SMAD4(D351H) antibody. HEK293T cells were transfected with pCDNA3-GFP SMAD4(D351H) plasmid , pCDNA3-GFP SMAD4(WT) plasmid or pCDNA3-GFP SMAD4(D351N) plasmid, then fixed and stained with anti SMAD4(D351H) monoclonal antibody (Cat. #26418).
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    Storage Conditions
    Store at -20°C. Avoid freeze / thaw cycles
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    Note
    For Research Use Only.