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- 产品名称： Anti RAS(A146V) Mouse Monoclonal Antibody
- 产品货号： CST26479
- 货期： 现货
- 价格与订购： 4800
- 数量：
库存： 5
- 规格： 100μg
- 产品信息
- 如何订购
Catalog Number
CST26479
Gene Symbol
H-Ras; K-Ras; N-Ras
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Description
Anti RAS(A146V) Mouse Monoclonal Antibody
Background
The A146V mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146V mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
Immunogen
A synthetic peptide from the internal region of Ras which includes the mutation of A146V, human origin.
Applications
ELISA, WB, IHC
Recommended Dilutions
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration
1 mg/ml
Host
mouse
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Format
Liquid
Clonality
Monoclonal
Purity
Purified from ascites
Preservative
no
Constituents
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity
Recognizes A146V mutant, but not wild-type RAS of vertebrates.
Immunoprecipitation/Western Blot
Western blot analysis of recombinant RAS(A146V) and wild-type proteins. Purified His-tagged RAS(A146V) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti RAS(A146V) monoclonal antibody (Cat. #26479).
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Immunofluorescence:
Immunofluorescence of cells expressing KRas proteins with anti KRAS(A146V) antibody. HEK293T cells were transfected with pCDNA3-GFP KRAS(A146V) plasmid, pCDNA3-GFP KRas(WT) plasmid, pCDNA3-GFP KRas(A146P) plasmid or pCDNA3-GFP KRas(A146T) plasmid, then fixed and stained with anti KRAS(A146V) monoclonal antibody (Cat. #26479).
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Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles
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Note
For Research Use Only.
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