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  • 产品名称: Anti RAS(A146V) Mouse Monoclonal Antibody
  • 产品货号: CST26479
  • 货期: 现货
  • 价格与订购: 4800
  • 数量:
    库存: 5
  • 规格: 100μg
  • 产品信息
  • 如何订购
    Catalog Number
    CST26479
    Gene Symbol
    H-Ras; K-Ras; N-Ras
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    Description
     Anti RAS(A146V) Mouse Monoclonal Antibody
    Background
    The A146V mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146V mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
    Immunogen
    A synthetic peptide from the internal region of Ras which includes the mutation of A146V, human origin.
    Applications
    ELISA, WB, IHC
    Recommended Dilutions
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration
    1 mg/ml
    Host
    mouse
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    Format
    Liquid
    Clonality
    Monoclonal
    Purity
    Purified from ascites
    Preservative
    no
    Constituents
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity
     Recognizes A146V mutant, but not wild-type RAS of vertebrates.
    Immunoprecipitation/Western Blot

    Western blot analysis of recombinant RAS(A146V) and wild-type proteins. Purified His-tagged RAS(A146V) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti RAS(A146V) monoclonal antibody (Cat. #26479).    
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    Immunofluorescence: 

    Immunofluorescence of cells expressing KRas proteins with anti KRAS(A146V) antibody. HEK293T cells were transfected with pCDNA3-GFP KRAS(A146V) plasmid, pCDNA3-GFP KRas(WT) plasmid, pCDNA3-GFP KRas(A146P) plasmid or pCDNA3-GFP KRas(A146T) plasmid, then fixed and stained with anti KRAS(A146V) monoclonal antibody (Cat. #26479).
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    Storage Conditions
    Store at -20°C. Avoid freeze / thaw cycles
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    Note
    For Research Use Only.