Anti RAS(A146V) Mouse Monoclonal Antibody
The A146V mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146V mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
A synthetic peptide from the internal region of Ras which includes the mutation of A146V, human origin.
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Recognizes A146V mutant, but not wild-type RAS of vertebrates.
Western blot analysis of recombinant RAS(A146V) and wild-type proteins. Purified His-tagged RAS(A146V) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti RAS(A146V) monoclonal antibody (Cat. #26479).
Immunofluorescence of cells expressing KRas proteins with anti KRAS(A146V) antibody. HEK293T cells were transfected with pCDNA3-GFP KRAS(A146V) plasmid, pCDNA3-GFP KRas(WT) plasmid, pCDNA3-GFP KRas(A146P) plasmid or pCDNA3-GFP KRas(A146T) plasmid, then fixed and stained with anti KRAS(A146V) monoclonal antibody (Cat. #26479).
Store at -20°C. Avoid freeze / thaw cycles