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  • 产品名称: Anti RAS(A146P) Mouse Monoclonal Antibody
  • 产品货号: CST26478
  • 货期: 现货
  • 价格与订购: 4800
  • 数量:
    库存: 5
  • 规格: 100μg
  • 产品信息
  • 如何订购
    Catalog Number
    CST26478
    Gene Symbol
    H-Ras; K-Ras; N-Ras
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    Description
    Anti RAS(A146P) Mouse Monoclonal Antibody
    Background
    The A146P mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146P mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
    Immunogen
    A synthetic peptide from the internal region of Ras which includes the mutation of A146P, human origin.
    Applications
     ELISA, IF, IHC
    Recommended Dilutions
    ELISA: 1:1000-1:5000 
    IF: 1:50-1:100 
    IHC: 1:50-1:100
    Concentration
    1 mg/ml
    Host
    mouse
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    Format
    Liquid
    Clonality
    Monoclonal
    Purity
    Purified from ascites
    Preservative
    no
    Constituents
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity
    Recognizes A146P mutant, but not wild-type RAS of vertebrates.    
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    Immunofluorescence: 

    Immunofluorescence of cells expressing KRas proteins with anti-KRAS(A146P) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRAS(A146P) plasmid , pCDNA3-GFP KRas(WT) plasmid, pCDNA3-GFP KRas(A146V) plasmid or pCDNA3-GFP KRas(A146T) plasmid, then fixed and stained with anti-KRAS(A146P) monoclonal antibody (Cat. #26478).
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    Storage Conditions
    Store at -20°C. Avoid freeze / thaw cycles
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    Note
    For Research Use Only.