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  • 产品名称: Anti RAS(G13V) Mouse Monoclonal Antibody
  • 产品货号: CST26477
  • 货期: 0
  • 价格与订购: 4800
  • 数量:
    库存: 5
  • 规格: 100μg
  • 产品信息
  • 如何订购
    Catalog Number
    CST26477
    Gene Symbol
    H-Ras; K-Ras; N-Ras
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    Description
     Anti RAS(G13V) Mouse Monoclonal Antibody
    Background
    Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13V mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
    Immunogen
     A synthetic peptide from the internal region of Ras which includes the mutation of G13V, human origin.
    Applications
     ELISA, WB, IHC
    Recommended Dilutions
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration
    1 mg/ml
    Host
    mouse
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    Format
    Liquid
    Clonality
    Monoclonal
    Purity
    Purified from ascites
    Preservative
    no
    Constituents
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity
     Recognizes G13V mutant, but not wild-type RAS of vertebrates.
    Immunoprecipitation/Western Blot

    Western blot analysis of recombinant Ras proteins. Purified His-tagged RAS(G13V) protein (lane 1), corresponding wildtype protein (lane 2), His-tagged Ras(G13S) protein (lane 3), His-tagged Ras(G13C) protein (lane 4), His-tagged Ras(G13R) protein (lane 5), His-tagged Ras(G13A) protein (lane 6) were blotted with Anti RAS(G13V) monoclonal antibody (Cat. #26477).   
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    Immunofluorescence: 


    Immunofluorescence of cells expressing Ras proteins with Anti RAS(G13V) antibody. HEK293T cells were transfected with pCDNA3-GFP-RAS(G13V) plasmid ,pCDNA3-GFP Ras(WT) plasmid, pCDNA3-GFP Ras(G13S) plasmid, pCDNA3-GFP Ras(G13C) plasmid, pCDNA3-GFP Ras(G13R) plasmid, pCDNA3-GFP Ras(G13A) plasmid or pCDNA3-GFP Ras(G13D) plasmid, then fixed and stained with Anti RAS(G13V) monoclonal antibody (Cat. #26477).
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    Storage Conditions
    Store at -20°C. Avoid freeze / thaw cycles
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    Note
    For Research Use Only.