Biotin-14-dATP is a dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker. The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids in in situ and filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP. Biotin-14-dATP can be added to the 3´ end of DNA using terminal deoxynucleotidyl transferase. The amount supplied is sufficient for labeling up to 50 µg of DNA by nick translation.
Incorporation into DNA/cDNA by
– Nick Translation with DNAse I/ DNA Polymerase I
– Primer Extension with Klenow fragment
For research use only. Not suitable for clinical or therapeutic use.