![]() |
Amily |
![]() |
Emily |
![]() |
Lily |
武汉科斯坦生物科技有限公司
-
在线咨询
-
官方邮箱
-
微信下单
-
回到顶部
代谢组学
科研服务
抗体/蛋白
地 址:武汉市东湖新技术开发区高新大道666号,武汉
国家生物产业基地B、C、D区研发楼C1栋
产品销售:15926423062
技术服务:18971263062
E - mail :info@chemstan.com
邮 编:430075
化合物单体
科斯坦生物(Chemstan)所有产品和服务仅用于科学研究,不为任何个人或者非科研性质的用途提供产品和服务。
Copyright @ 武汉科斯坦生物科技有限公司.All rights reserved
网站建设:中企动力武汉鄂ICP备19006850号-1
浏览量:
产品名称:
Eliglustat 依利格鲁司特
NO.
69
货号
CST3663
中文名称
依利格鲁司特
英文名称
Eliglustat
CAS号
491833-29-5
产品货号:
CST3663
货期:
现货
价格与订购:
1280.00
数量
-
+
库存
5
产品信息
如何订购
产品描述icon
Eliglustat is an oral inhibitor of glucosylceramide synthase which is used in the therapy of type 1 Gaucher disease.
体外活性icon
Eliglustat Tartrate啮齿类动物体内快速代谢,其体内半衰期在15-45分钟.对正常小鼠、大鼠、狗中通过静脉注射和口服途径给药(Eliglustat Tartrate),脾脏、肾脏和肝脏中葡糖神经酰胺的含量显著下降.
体内活性icon
Eliglustat tartrate是葡萄糖神经酰胺合成酶的抑制剂,对该酶的抑制作用高度特异,对其他多种糖苷酶的抑制作用有限甚至没有抑制活性。
激酶实验
icon
icon
Smo-binding assays are conducted with BODIPY-cyclopamine and Smo-overexpressing HEK 293T cells, using a CMVpromoter-based SV40 origin-containing expression construct for Smo-Myc3 (murine Smo containing three consecutive Myc epitopes at the C terminus). HEK 293T cells are seeded into eight-well chambered coverslips (80,000 cells/well) and cultured in DMEM containing 10% FBS, 100 U/mL penicillin, and 0.1 mg/mL streptomycin. The cells are cultured until they reached 55 to 65% confluency (14-18 h), after which they are transfected with the Smo-Myc3 expression construct and Transit-LT1. Twenty-four hours after transfection, the cells are washed with PBS and cultured in DMEM containing 0.5% FBS, 5 nM BODIPY-cyclopamine, and various concentrations of either cyclopamine or individual HPIs. After 30 min, 10 μM Hoescht 33342 is added to each well, and the HPIs are incubated with the cells for an additional 30 min. The cells are then washed two times with PBS buffer, once with phenol red-free DMEM containing 0.5% FBS, and immediately imaged using a DMI6000B compound microscope. Images are background-substracted using ImageJ software with a rolling ball size of 75 pixels, and BODIPY-cyclopamine intensity is then determined using Metamorph software. Circular regions with a diameter of 300 pixels are placed over regions containing uniformly confluent cells, and the pixel intensities of approximately 20 regions from four independent images is used to determine the average BODIPY-cyclopamine levels for each experimental condition[1].
细胞实验icon
The inhibitory activity of Genz-112638 is determined indirectly by measuring its effect on the cell surface levels of the gangliosides GM1 and GM3 on either K562 or B16/F10 cells. GM1 levels on the K562 cells are determined by incubating the cells with increasing amounts of Genz-112638 (0.6-1000 nM) for 72 h after which the cells are harvested and stained using 10 μg of recombinant cholera toxin-FITC in 100 μL phosphate buffered saline (PBS) containing 0.5% bovine serum albumin (BSA) for 30 min on ice. Cells are ished, resuspended in PBS containing 0.5% BSA and the fluorescence quantitated[1].
分子式icon
C23H36N2O4
分子量icon
404.551
别名icon
依利格鲁司特;GENZ-112638;Genz 99067
参考文献icon
[1]Shayman JA. Drugs Future. 2010, 35(8):613-620.
[2]Nelson S. Yew, et al. PLoS One. 2010, 5(6):e11239.icon
储存和溶解度
icon
icon
DMSO:74 mg/mL (182.9 mM)
H2O:<1 mg/mL
Ethanol:74 mg/mL (182.9 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
icon
Note
icon
icon
For Research Use Only.
上一篇
Leucocyanidin 白矢车菊素
Eliglustat hemitartrate 依利格鲁司特酒石酸盐
下一篇