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- 产品名称： Recombinant Human POLD1 protein
- 产品货号： CSP01926
- 货期： 现货
- 价格与订购： 1800
- 数量：
库存： 100
- 规格： 50μg 100μg 1mg
- 产品信息
- 如何订购
概述（Summary）
英文全称
Recombinant Human POLD1 protein
纯度（Purity）
>90% as determined by SDS-PAGE
内毒素（Endotoxin level）
Please contact with the lab for this information.
蛋白构建（Construction）
A DNA sequence encoding the human POLD1 (Met41-Phe254) was fused with GST tag
Accession #
P28340
表达宿主（Host）
E.coli
种属（Species）
Homo sapiens (Human)
预测分子量（Predicted Molecular Mass）
48.36 kDa
制剂（Formulation）
Supplied as solution form in PBS pH 7.5 or lyophilized from PBS pH 7.5.
运输方式（Shipping）
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性&储存（Stability &Storage）
Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
复溶（Reconstitution）
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
背景（Background）
背景介绍
As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated . Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation . Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites .
分子别名（Alternative Names）
DNA polymerase delta catalytic subunit,3'-5' exodeoxyribonuclease,DNA polymerase subunit delta p125.
Note
For research use only .
地 址：
产品销售：
E - mail ：
邮 编：
Amily
