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  • 产品名称: Apolipoprotein E3 Protein
  • 产品货号: TD-P-1002
  • 货期: 现货
  • 价格与订购: 2800
  • 数量:
    库存: 5
  • 规格: 50μg
  • 产品信息
  • 如何订购
    Catalog No.
    TD-P-1002  
    Protein Name
     APOE 3
    Uniprot No
     P02649
    Expression System
    E. Coli
    Compound Identity
    APOE3(e1)-his
    Full Length or Truncated
    Full length
    Molecular Weight
    36.7 kDa
    Form
    freeze-dried powder
    Storage
    -80°C (as supplied)
    Stability
    ≥6 months
    Purity
    ≥70% estimated by SDS-PAGE
    Supplied in
    1×PBS (pH 7.2)
    Description
    ApoE is a member of the superfamily of amphiphilic exchangeable apolipoproteins and a lipid carrier protein with a major role in lipid homeostasis.1,2 It is expressed in astrocytes, hepatocytes, monocytes, macrophages, and kidney cells and exists as three major polymorphic alleles, ApoE2, ApoE3, and ApoE4, which occur in the United States population with frequencies of approximately 7, 79, and 14%, respectively. ApoE is composed of an N-terminal domain, which contains sequences for binding to members of the LDL receptor family, and a C-terminal domain, containing the major lipid-binding region, linked by an unstructured hinge region, which facilitates domain mobility essential to protein function.1 Upon lipid binding, ApoE undergoes a conformational change that orients the α-helices of the C-terminal domain perpendicular to the acyl chains of the bound lipids to stabilize the bound lipids and facilitates recognition and binding to LDL receptors by the N-terminal domain. ApoE isoforms vary at amino acids 130 and 176 (112 and 158, respectively, in the mature protein) with ApoE2 containing cysteine at 130 and 176, ApoE3 containing cysteine at 130 and arginine at 176, and ApoE4 containing arginine at 130 and 176. ApoE3 forms homodimers via disulfide bond formation. ApoE3 is more efficient than ApoE4, but less efficient than ApoE2, at promoting cholesterol efflux in astrocytes, and APOE3-expressing astrocytes accumulate fewer but larger lipid droplets than astrocytes expressing APOE4. 3,4 Unlike the increased risk of Alzheimer’s disease associated with the ApoE4 variant, ApoE3 is neutral with respect to Alzheimer’s disease risk, and ApoE3 with a valine-to-glutamine substitution at position 236 (ApoE3V236E) is associated with a reduce risk for the disease.5 Cayman’s ApoE3 (human, recombinant) protein can be used for cell-based assays.
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    Note
    For research use only.