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  • 产品名称: Protein A ELISA Kit
  • 产品货号: BK301
  • 货期: 现货
  • 价格与订购: 3800
  • 数量:
    库存: 5
  • 规格: 96T
  • 产品信息
  • 如何订购
    Intended Use 
    Bioyeargene Protein A ELISA Kit is intended for use in quantitating unnatural protein A constructs such as Recombinant Alkali-tolerant Protein A. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. This kit is designed to provide a validated acidifing sample treatment method to dissociate Protein A from IgG products and detect Protein A contamination to less than 78pg/mL in the presence of up to 3mg/mL quantities of humanized monoclonal antibodies.
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    Principle of the Assay
    Protein A ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Protein A. Samples containing Protein A  are  first  diluted  in  the  Sample Diluent provided with the kits. The Denaturing Buffer is then added and  mixed to dissociate the Protein A from the product antibody. The samples are then reacted in microtiter strips coated with a monoclonal anti-Protein A capture antibody.  A second anti-Protein A  biotinylated monoclonal antibody is simultaneously reacted forming a sandwich complex of solid phase antibody-Protein A:biotin labeled antibody. After a wash step to remove any unbound reactants, Streptavidin labeled HRP is added to each well. After a short incubation period, the strips are then reacted with TMB substrate. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Protein A concentration in each sample is interpolated from this standard curve. 
    Materials & Equipment Required But Not Provided
    1. Precision pipettors and disposable tips to deliver 10-1000 μl. A multi-channel   
    pipette is desirable for large assays.
    2. 100 mL and 1 liter graduated cylinders.
    3. Distilled or deionized water.
    4. Tubes to prepare sample dilutions.
    5. Microplate reader capable of measuring absorbance at 450 nm.
    6. Data analysis and graphing software. 
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    Note
    For Research Use Only.