![]() |
Amily |
![]() |
Emily |
![]() |
Lily |
武汉科斯坦生物科技有限公司
-
在线咨询
-
官方邮箱
-
微信下单
-
回到顶部
代谢组学
科研服务
抗体/蛋白
地 址:武汉市东湖新技术开发区高新大道666号,武汉
国家生物产业基地B、C、D区研发楼C1栋
产品销售:15926423062
技术服务:18971263062
E - mail :info@chemstan.com
邮 编:430075
化合物单体
科斯坦生物(Chemstan)所有产品和服务仅用于科学研究,不为任何个人或者非科研性质的用途提供产品和服务。
Copyright @ 武汉科斯坦生物科技有限公司.All rights reserved
网站建设:中企动力武汉鄂ICP备19006850号-1
浏览量:
产品名称:
Rab7 Activation Assay Kit
NO.
95
货号:
82501
中文名称
Rab7 Activation Assay Kit
英文名词
Rab7 Activation Assay Kit
CAS号
产品货号:
82501
货期:
现货
价格与订购:
6800.00
数量:
-
+
库存:
5
产品信息
如何订购
Product Description
icon
icon
Rab7 belongs to the Rab family of small GTPases. Rab7 is located in the late endosome, lysosome,cytoplasmic vesicle, phagosome and melanosome. It is involved in the late endocytic transport and contributes to the maturation of phagosomes.
Currently there is no direct assay to measure the activation of Rab7 GTPases.
Bioyears Biosciences Rab7 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab7 GTP, but not Rab7 GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
Currently there is no direct assay to measure the activation of Rab7 GTPases.
Bioyears Biosciences Rab7 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab7 GTP, but not Rab7 GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
These anti Rab7-GTP monoclonal antibodies can also be used to monitor the activation of Rab7 in cells and in tissues by immunohistochemistry.
Bioyears Biosciences Rab7 Activation Assay Kit provides a simple and fast method to monitor the activation of Rab7. Each kit provides sufficient quantities to perform 20 assays.
iconiconiconicon
Assay Principleicon
Bioyears Biosciences Rab7 Activation Assay Kit bases on the configuration-specific anti Rab7 GTP monoclonal antibody to measure the active Rab7 GTP levels, either from cell extracts or from in vitro GTPγS loading Rab7 activation assays. Briefly, anti active Rab7 mouse monoclonal antibody will be incubated with cell lysates containing Rab7 GTP. The bound active Rab7 will then be pulled down by protein A/G agarose. The precipitated active Rab7 will be detected by immunoblot analysis using antiRab7 rabbit polyclonal antibody.
Kit Componentsicon
1. Anti active Rab7, Mouse Monoclonal Antibody (Catalog No. 26923): One vial – 22 µL (1
mg/ml) in PBS, pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibody
specifically recognizes Rab7 GTP from all vertebrates.
2. Protein A/G Agarose (Catalog No. 30301): One vial – 400 µL of 50% slurry.
3. 5X Assay/Lysis Buffer (Catalog No. 30302): One bottle – 30 mL of 250 mM Tris-HCl, pH 8, 750 mM NaCl, 50 mM MgCl2, 5 mM EDTA, 5% Triton X-100.
4. Anti Rab7, Rabbit Polyclonal Antibody (Catalog No. 21069): One vial – 100 µL (1 mg/ml)
in PBS, pH 7.4, contained 50% glycerol.
5. 100 X GTPγS (Catalog No. 30303): One vial –100 l at 10 mM, use 5 µL of GTPγS for
GTP-labeling of 0.5 mL of cell lysate.
6. 100 X GDP (Catalog No. 30304): One vial –100 l at 100 mM, use 5 µL of GDP for
GDP-labeling of 0.5 mL of cell lysate.
iconicon
Storageicon
Store all kit components at 4ºC until their expiration dates.
iconiconicon
Noteicon
For Research Use Only.
上一篇
Rap1 Activation Assay Kit
Rab5A Activation Assay Kit
下一篇