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- 产品名称： Rab4A Activation Assay Kit
- 产品货号： 82701
- 货期： 现货
- 价格与订购： 6800
- 数量：
库存： 5
- 规格： 96T
- 产品信息
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Product Description
Rab-4A belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20,
RUFY1, SGSM1, SGSM2, SGSM3 and other proteins. It participates in protein transport and in
vesicular traffic.
Currently there is no direct assay to measure the activation of Rab4A GTPases.
Bioyears Biosciences Rab4A Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab4A GTP, but not Rab4A GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
These anti Rab4A GTP monoclonal antibodies can also be used to monitor the activation of Rab4A in cells and in tissues by immunohistochemistry.
Bioyears Biosciences Rab4A Activation Assay Kit provides a simple and fast method to monitor the activation of Rab4A. Each kit provides sufficient quantities to perform 20 assays.
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Assay Principle
Bioyears Biosciences Rab4A Activation Assay Kit bases on the configuration-specific anti Rab4A GTP monoclonal antibody to measure the active Rab4A GTP levels, either from cell extracts or from in vitro GTPγS loading Rab4A activation assays. Briefly, anti active Rab4A mouse monoclonal antibody will be incubated with cell lysates containing Rab4A GTP. The bound active Rab4A will then be pulled down by protein A/G agarose beads. The precipitated active Rab4A will be detected by immunoblot analysis using anti Rab4A rabbit polyclonal antibody.
Kit Components
1. Anti active Rab4A, Mouse Monoclonal Antibody (Catalog No. 26921): One vial – 22 µL (1
mg/ml) in PBS, pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibody
specifically recognizes Rab4A GTP from all vertebrates.
2. Protein A/G Agarose (Catalog No. 30301): One vial – 400 µL of 50% slurry.
3. 5X Assay/Lysis Buffer (Catalog No. 30302): One bottle – 30 mL of 250 mM Tris-HCl, pH 8, 750 mM NaCl, 50 mM MgCl2, 5 mM EDTA, 5% Triton X-100.
4. Anti Rab4A, Rabbit Polyclonal Antibody (Catalog No. 21158): One vial – 100 µL (1 mg/ml)
in PBS, pH 7.4, contained 50% glycerol.
5. 100 X GTPγS (Catalog No. 30303): One vial –100 μl at 10 mM, use 5 µL of GTPγS for
GTP-labeling of 0.5 mL of cell lysate.
6. 100 X GDP (Catalog No. 30304): One vial –100 μl at 100 mM, use 5 µL of GDP for
GDP-labeling of 0.5 mL of cell lysate.
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Storage
Store all kit components at 4ºC until their expiration dates.
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Note
For Research Use Only.
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