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Rab4A Activation Assay Kit

NO.
91
货号:
82701
中文名称
Rab4A Activation Assay Kit
英文名词
Rab4A Activation Assay Kit
CAS号
产品货号:
82701
货期:
现货
价格与订购:
6800.00
数量
-
+
库存:
5
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Product Description
icon
 Rab-4A belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20,
RUFY1, SGSM1, SGSM2, SGSM3 and other proteins. It participates in protein transport and in
vesicular traffic.
Currently there is no direct assay to measure the activation of Rab4A GTPases.
Bioyears Biosciences Rab4A Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab4A GTP, but not Rab4A GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
These anti Rab4A GTP monoclonal antibodies can also be used to monitor the activation of Rab4A in cells and in tissues by immunohistochemistry.
Bioyears Biosciences Rab4A Activation Assay Kit provides a simple and fast method to monitor the activation of Rab4A. Each kit provides sufficient quantities to perform 20 assays. 
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Assay Principleicon
Bioyears Biosciences Rab4A Activation Assay Kit bases on the configuration-specific anti Rab4A GTP monoclonal antibody to measure the active Rab4A GTP levels, either from cell extracts or from in vitro GTPγS loading Rab4A activation assays. Briefly, anti active Rab4A mouse monoclonal antibody will be incubated with cell lysates containing Rab4A GTP. The bound active Rab4A will then be pulled down by protein A/G agarose beads. The precipitated active Rab4A will be detected by immunoblot analysis using anti Rab4A rabbit polyclonal antibody. 
Kit Componentsicon
1. Anti active Rab4A, Mouse Monoclonal Antibody (Catalog No. 26921): One vial – 22 µL (1
    mg/ml) in PBS, pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibody
    specifically recognizes Rab4A GTP from all vertebrates.
2. Protein A/G Agarose (Catalog No. 30301): One vial – 400 µL of 50% slurry.
3. 5X Assay/Lysis Buffer (Catalog No. 30302): One bottle – 30 mL of 250 mM Tris-HCl, pH 8,     750 mM NaCl, 50 mM MgCl2, 5 mM EDTA, 5% Triton X-100.
4. Anti Rab4A, Rabbit Polyclonal Antibody (Catalog No. 21158): One vial – 100 µL (1 mg/ml)
    in PBS, pH 7.4, contained 50% glycerol.
5. 100 X GTPγS (Catalog No. 30303): One vial –100 μl at 10 mM, use 5 µL of GTPγS for
    GTP-labeling of 0.5 mL of cell lysate.
6. 100 X GDP (Catalog No. 30304): One vial –100 μl at 100 mM, use 5 µL of GDP for
    GDP-labeling of 0.5 mL of cell lysate. 
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Storageicon
Store all kit components at 4ºC until their expiration dates. 
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Noteicon
For Research Use Only.
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