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- 产品名称： Rab3 Activation Assay Kit
- 产品货号： 82601
- 货期： 现货
- 价格与订购： 6800
- 数量：
库存： 5
- 规格： 96T
- 产品信息
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Product Description
Rab3 is a member of the Rab-family GTPases and is specifically targeted to synaptic vesicles
Currently there is no direct assay to measure the activation of Rab3 GTPases.
Bioyears Biosciences Rab3 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab3 GTP, but not Rab3 GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
These anti Rab3 -GTP monoclonal antibodies can also be used to monitor the activation of Rab3 in cells and in tissues by immunohistochemistry.
Bioyears Biosciences Rab3 Activation Assay Kit provides a simple and fast method to monitor the activation of Rab3. Each kit provides sufficient quantities to perform 20 assays.
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Assay Principle
Bioyears Biosciences Rab3 Activation Assay Kit bases on the configuration-specific anti Rab3 GTP monoclonal antibody to measure the active Rab3 GTP levels, either from cell extracts or from in vitro GTPγS loading Rab3 activation assays. Briefly, anti active Rab3 mouse monoclonal antibody will be incubated with cell lysates containing Rab3 GTP. The bound active Rab3 will then be pulled down by protein A/G agarose. The precipitated active Rab3 will be detected by immunoblot analysis using antiRab3 rabbit polyclonal antibody.
Kit Components
1. Anti active Rab3, Mouse Monoclonal Antibody (Catalog No. 26920): One vial – 22 µL (1
mg/ml) in PBS, pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibody
specifically recognizes Rab3 GTP from all vertebrates.
2. Protein A/G Agarose (Catalog No. 30301): One vial – 400 µL of 50% slurry.
3. 5X Assay/Lysis Buffer (Catalog No. 30302): One bottle – 30 mL of 250 mM Tris-HCl, pH 8, 750
mM NaCl, 50 mM MgCl2, 5 mM EDTA, 5% Triton X-100.
4. Anti Rab3A, Rabbit Polyclonal Antibody (Catalog No. 21041): One vial – 100 µL (1 mg/ml)
in PBS, pH 7.4, contained 50% glycerol.
5. 100 X GTPγS (Catalog No. 30303): One vial –100 µl at 10 mM, use 5 µL of GTPγS for
GTP-labeling of 0.5 mL of cell lysate.
6. 100 X GDP (Catalog No. 30304): One vial –100 µl at 100 mM, use 5 µL of GDP for
GDP-labeling of 0.5 mL of cell lysate.
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Storage
Store all kit components at 4ºC until their expiration dates.
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Note
For Research Use Only.
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