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  • 产品名称: CD3 epsilon Rabbit mAb
  • 产品货号: CSTR23808
  • 货期: 现货
  • 价格与订购: 2200
  • 数量:
    库存: 5
  • 规格: 100μg
  • 产品信息
  • 如何订购
    Background
    Swiss-Prot Acc.P07766.Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).
    Alternative Names
    T3E; TCRE; IMD18
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    Immunogen
    A synthetic peptide of human CD3 epsilon
    Cellular localization
    Cell membrane, Membrane
    Immunogen
    A synthetic peptide from the C-terminal region of RhoA.
    Cellular localization
    Cell membrane, Membrane
    Buffer System
    50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
    Dilution RatioWB
    1/1000-1/5000 IHC: 1/50-1/200 IP: 1/20
    The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma,
    -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex.
    This complex plays an important role in coupling antigen recognition to
    Gene Description
    several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the
    same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause
    immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
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    Molecular Weight
    Calculated MW: 23 kDa; Observed MW: 23 kDa
    Gene Name
    CD3E
    Host
    Rabbit
    Reactivity
    Human
    Conjugation
    Unconjugated
    Application
    WB,IHC-P,IP
    Storage
    Store at 4℃ short term. Aliquot and store at -20℃ long term. Avoid freeze/thaw cycles.
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    SwissProtID
    P07766
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    Purification
    Affinity Purified
    Clonality No
    R02-1B5
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    Clonality
    Monoclonal antibody
    Isotype
    Rabbit IgG
    Antibody
    TypePrimary Antibody
    Research
    FieldImmunology
    Gene ID
    916

    Western blot detection of CD3 epsilon in Jurkat cell lysates using CD3 epsilon
    Rabbit mAb(1:500 diluted).Predicted band size:23kDa.Observed band size:23kDa.

    Immunohistochemistry of CD3 epsilon in paraffin-embedded Human tonsil 
    using CD3 epsilon Rabbit mAb at dilution 1/50
    Note
    For research use only .icon