Anti PIK3CA(E545G) Mouse Monoclonal Antibody

CST26208

PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha

iconicon

 Anti PIK3CA(E545G) Mouse Monoclonal Antibody

Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.

A synthetic peptide from the internal region of PIK3CA which includes the mutation of E545G, human origin.

ELISA, WB, IHC

1 mg/ml

mouse

icon

Liquid

Monoclonal

Purified from ascites

no

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Recognizes E545G mutant, but not wild-type PIK3CA of vertebrates.

Western blot analysis of recombinant PIK3CA(E545G) and wildtype proteins. Purified His-tagged PIK3CA(E545G) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti PIK3CA(E545G) monoclonal antibody (Cat. #26208).        
icon

Immunofluorescence of cells expressing PIK3CA proteins with Anti PIK3CA(E545G) antibody. HEK293T cells were transfected with pCDNA3-GFP PIK3CA(WT) plasmid (left column) or pCDNA3-GFP PIK3CA(E545G) plasmid (right column), then fixed and stained with Anti PIK3CA(E545G) monoclonal antibody (Cat. #26208).

iconicon

Store at -20°C. Avoid freeze / thaw cycles

iconiconicon

For Research Use Only.