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武汉科斯坦生物科技有限公司
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代谢组学
科研服务
抗体/蛋白
地 址:武汉市东湖新技术开发区高新大道666号,武汉
国家生物产业基地B、C、D区研发楼C1栋
产品销售:15926423062
技术服务:18971263062
E - mail :info@chemstan.com
邮 编:430075
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产品名称:
Anti PIK3CA(E545G) Mouse Monoclonal Antibody
NO.
货号:
CST26208
中文名称
Anti PIK3CA(E545G) Mouse Monoclonal Antibody
英文名词
Anti PIK3CA(E545G) Mouse Monoclonal Antibody
CAS号
产品货号:
CST26208
货期:
现货
价格与订购:
4800.00
数量:
-
+
库存:
5
产品信息
如何订购
Catalog Numbericon
CST26208
Gene Symbolicon
PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
iconicon
Descriptionicon
Anti PIK3CA(E545G) Mouse Monoclonal Antibody
Backgroundicon
Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
Immunogenicon
A synthetic peptide from the internal region of PIK3CA which includes the mutation of E545G, human origin.
Applicationsicon
ELISA, WB, IHC
Recommended Dilutionsicon
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentrationicon
1 mg/ml
Hosticon
mouse
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Formaticon
Liquid
Clonalityicon
Monoclonal
Purityicon
Purified from ascites
Preservativeicon
no
Constituentsicon
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivityicon
Recognizes E545G mutant, but not wild-type PIK3CA of vertebrates.
Immunoprecipitation/Western Blot
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icon

Western blot analysis of recombinant PIK3CA(E545G) and wildtype proteins. Purified His-tagged PIK3CA(E545G) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti PIK3CA(E545G) monoclonal antibody (Cat. #26208).
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Immunofluorescence: icon

Immunofluorescence of cells expressing PIK3CA proteins with Anti PIK3CA(E545G) antibody. HEK293T cells were transfected with pCDNA3-GFP PIK3CA(WT) plasmid (left column) or pCDNA3-GFP PIK3CA(E545G) plasmid (right column), then fixed and stained with Anti PIK3CA(E545G) monoclonal antibody (Cat. #26208).
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Storage Conditionsicon
Store at -20°C. Avoid freeze / thaw cycles
iconiconicon
Noteicon
For Research Use Only.
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