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武汉科斯坦生物科技有限公司
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Wuhan Chemstan Biotechnology Co., Ltd.
武汉科斯坦生物科技有限公司
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产品名称：
Anti Active Rac Mouse Monoclonal Antibody
NO.
货号：
CST26903
中文名称
Anti Active Rac1-GTP Mouse Monoclonal Antibody
英文名词
Anti Active Rac1-GTP Mouse Monoclonal Antibody
CAS号
产品货号：
CST26903
货期：
现货
价格与订购：
4800.00
数量：
-
+
库存：
5
产品信息
如何订购
Catalog Numbericon
CST26903
Gene Symbolicon
RAC1
iconicon
Descriptionicon
Anti Active Rac1-GTP Mouse Monoclonal Antibody
Backgroundicon
Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
Immunogenicon
Recombinant full length protein of active Rac1
Applicationsicon
IP, IHC
Recommended Dilutionsicon
1 µg for 1~2 mg total cellular proteins for IP;
1:100 dilution for IHC
Concentrationicon
1 mg/ml
Hosticon
mouse
icon
Formaticon
Liquid
Clonalityicon
Monoclonal
Isotypeicon
IgM
Purityicon
Purified from ascites
Preservativeicon
no
Constituentsicon
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Rac1 antibody recognizes active Rac1 from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Species Reactivityicon
Anti-active Rac1 antibody recognizes active Rac1 from vertebrates.
Immunoprecipitation/Western Blot
icon
icon
Rac activation assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti active Rac monoclonal antibody (Cat # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti Rac rabbit polyclonal antibody (Cat # 21003). The bottom panel shows the Western blot with anti Rac of the cell lysates used (5% of that used in the top panel).
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Storage Conditionsicon
Store at -20°C. Avoid freeze / thaw cycles
iconicon
Immunofluorescence:icon
Immunofluorescence for the active Rac using Anti Active Rac1 GTP Mouse Monoclonal Antibody [26903] shows Rac GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections.
The tissue sections were fixed with -20 ºC methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti mouse AlexaFluor488 and anti rabbit AlexaFluor594 at 1:1000.
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Noteicon
For Research Use Only.
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