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  • 产品名称: Anti Active Rac Mouse Monoclonal Antibody
  • 产品货号: CST26903
  • 货期: 现货
  • 价格与订购: 4800
  • 数量:
    库存: 5
  • 规格: 100μg
  • 产品信息
  • 如何订购
    Catalog Number
    CST26903
    Gene Symbol
     RAC1
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    Description
    Anti Active Rac1-GTP Mouse Monoclonal Antibody
    Background
    Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
    Immunogen
    Recombinant full length protein of active Rac1
    Applications
    IP, IHC
    Recommended Dilutions
    1 µg for 1~2 mg total cellular proteins for IP; 
     1:100 dilution for IHC
    Concentration
    1 mg/ml
    Host
    mouse
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    Format
    Liquid
    Clonality
    Monoclonal
    Isotype
    IgM
    Purity
    Purified from ascites
    Preservative
    no
    Constituents
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Rac1 antibody recognizes active Rac1 from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Species Reactivity
    Anti-active Rac1 antibody recognizes active Rac1 from vertebrates.
    Immunoprecipitation/Western Blot

    Rac activation assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti active Rac monoclonal antibody (Cat # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti Rac rabbit polyclonal antibody (Cat # 21003). The bottom panel shows the Western blot with anti Rac of the cell lysates used (5% of that used in the top panel).                                                 
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    Storage Conditions
    Store at -20°C. Avoid freeze / thaw cycles
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    Immunofluorescence:

    Immunofluorescence for the active Rac using Anti Active Rac1 GTP Mouse Monoclonal Antibody [26903] shows Rac GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. 
    The tissue sections were fixed with -20 ºC methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti mouse AlexaFluor488 and anti rabbit AlexaFluor594 at 1:1000.
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    Note
    For Research Use Only.