Anti Active Ras Mouse Monoclonal Antibody
Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
Recombinant full length protein of active H-Ras
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
nti active Ras antibody recognizes active H-Ras, N-Ras, and K-Ras from vertebrates.
IP/WB analysis of active Ras protein. Purified recombinant Ras proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti active Ras mouse monoclonal antibody (Cat. # 26909). After SDS/PAGE, the membrane filter was probed with anti Ras mouse monoclonal antibody (Cat. # 26014).
Store at -20°C. Avoid freeze / thaw cycles