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  • 产品名称: Anti Active RhoA Mouse Monoclonal Antibody
  • 产品货号: CS26904
  • 货期: 现货
  • 价格与订购: 2400
  • 数量:
    库存: 5
  • 规格: 30UL
  • 产品信息
  • 如何订购
    Catalog Number
    CS26904
    Description
    Anti Active RhoA Mouse Monoclonal Antibody
    Background
    Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction.
    Immunogen
    Recombinant full length protein of active RhoA
    Applications
    IP, IHC
    Recommended Dilutions
    1 µg for 1~2 mg total cellular proteins for IP; 
    1:100 dilution for IHC
    Concentration
    1 mg/ml
    Host
    mouse
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    Format
    Liquid
    Clonality
    Monoclonal
    Isotype
    IgG2b
    Purity
    Purified from ascites
    Preservative
    no
    Constituents
    PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity
    Anti-active RhoA antibody recognizes active RhoA from vertebrates.
    Immunoprecipitation/Western Blot
         
    IP/WB analysis of active RhoA protein. Purified recombinant RhoA proteins wer
    e loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated
    with anti active RhoA mouse monoclonal antibody (Cat. # 26904). After SDS/PAGE,
    membrane filter was probed with anti RhoA mouse monoclonal antibody (Cat. # 26007).                                                                                                        
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    Storage Conditions
    Store at -20°C. Avoid freeze / thaw cycles
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    Note
    For Research Use Only.