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产品名称：
邻硝基苯-β-D-吡喃半乳糖苷 ONPG
NO.
5
货号
CST19497
中文名称
2-硝基苯-beta-D-半乳糖苷
英文名称
ONPG
CAS号
369-07-3
产品货号：
CST19497
货期：
现货
价格与订购：
285.00
数量
-
+
库存
20
产品信息
如何订购
产品描述icon
ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.
靶点活性icon
Others
体外活性icon
To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8/s and 2.7±0.3 mM for ONPG, and 264.2±2.1/s and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 /s/mM) is 4.6-fold higher than that for lactose (37.2 /s/mM), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose[1]. Gal308 displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity[1].
别名icon
2-Nitrophenyl β-D-galactopyranoside , 2-硝基苯-beta-D-半乳糖苷
纯度icon
99.79%
分子量icon
301.25
分子式icon
C12H15NO8
CAS Noicon
369-07-3
外观icon
White or white-like powder
溶液颜色(1%水溶液)icon
Colorless to light yellowicon
存储icon
Powder: -20°C for 3 years In solvent: -80°C for 2 years
溶解度icon
H2O: 7.4 mg/mL (24.56 mM), Need ultrasonic ( < 1 mg/ml refers to the product slightly soluble or insoluble )
参考文献icon
1. Zhang X, et al. Metagenomic approach for the isolation of a thermostable β-galactosidase with high tolerance of galactose and glucose from soil samples of Turpan Basin. BMC Microbiol. 2013 Oct 24;13:237.
Noteicon
For research use only .
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