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  • 产品名称: 邻硝基苯-β-D-吡喃半乳糖苷 ONPG
  • 产品货号: CST19497
  • 货期: 现货
  • 价格与订购: 285
  • 数量:
    库存: 10
  • 规格: 5g 25g
  • 产品信息
  • 如何订购
    产品描述
    ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.
    靶点活性
    Others 
    体外活性
    To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8/s and 2.7±0.3 mM for ONPG, and 264.2±2.1/s and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 /s/mM) is 4.6-fold higher than that for lactose (37.2 /s/mM), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose[1]. Gal308 displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity[1].
    别名
    2-Nitrophenyl β-D-galactopyranoside , 2-硝基苯-beta-D-半乳糖苷
    纯度
    99.79% 
    分子量
    301.25 
    分子式
    C12H15NO8 
    CAS No
    369-07-3 
    外观
    White or white-like powder
    溶液颜色(1%水溶液)
    Colorless to light yellowicon
    存储
    Powder: -20°C for 3 years   In solvent: -80°C for 2 years
    溶解度
    H2O: 7.4 mg/mL (24.56 mM), Need ultrasonic  ( < 1 mg/ml refers to the product slightly soluble or insoluble )
    参考文献
    1. Zhang X, et al. Metagenomic approach for the isolation of a thermostable β-galactosidase with high tolerance of galactose and glucose from soil samples of Turpan Basin. BMC Microbiol. 2013 Oct 24;13:237.
    Note
    For research use only .