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  • 产品名称: FG-4592
  • 产品货号: CS2515
  • 货期: 0
  • 价格与订购: 2000
  • 数量:
    库存: 100
  • 规格: 1g 10mg 100mg
  • 产品信息
  • 如何订购
    产品描述
    Roxadustat is an orally bioavailable, hypoxia-inducible factor prolyl hydroxylase inhibitor (HIF-PHI), with potential anti-anemic activity. 
    靶点活性
    HIF-PH
    体内活性
    FG-4592 (25 mg/kg) enhances mitophagy and attenuates retinal histopathologic damage in the photoreceptor layer after RD in an RD animal model[1]. 
    细胞实验
    The left eye of each rat is retro-orbitally injected with 25 mg/kg FG-4592 every 2 days, and an equal volume of 20% DMSO diluted with 0.9% NaCl is administrated with retro-orbital injection and served as the control. The retinae (attached, 1 day, 3 days, 5 days, and 7 days after RD) are homogenized and lysed with buffer containing 50 mM hydroxymethyl(Tris)-aminomethane(HCl), 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, and a protease inhibitor tablet. Samples are run on 8% to 12% 2-hydroxyethyl (Bis)-hydroxymethyl (Tris) gel electrophoresis and transferred onto polyvinylidene difluoride (PVDF) membranes (0.2-mm pores). After blocking with 3% nonfat dried milk, the membranes are incubated overnight with primary antibody HIF-1α, LC3, BNIP3, autophagy-related gene 5 (Atg5), and β-actin. The blotted membranes are then incubated for 60 minutes at room temperature with a horseradish peroxidase (HRP)-labeled secondary antibody. Immunoreactive bands are visualized by enhanced chemiluminescence (ECL) and detected with an Amersham Imager 600. A minimum of three rats are used for each condition.  icon
    别名
    FG-4592 , ASP1517 
    纯度
    >99% 
    分子量
    352.34
    分子式
    C24H27F3N8O3
    CAS No
    808118-40-3
    存储
    Powder: -20°C for 3 years
    In solvent: -80°C for 6 months
    溶解度
    DMSO: 65 mg/mL (184.5 mM)
    Ethanol: <1 mg/mL
    Water: <1 mg/mL
    ( < 1 mg/ml refers to the product slightly soluble or insoluble )
    icon
    Note
    For research use only .